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KMID : 0364519960080010083
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Dong-A Journal Medicine
1996 Volume.8 No. 1 p.83 ~ p.90
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Experimental Study of Main Neurotransmitter Involved in Rabbit Cavernous Smooth Muscle Relaxation
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정경우. 김춘곤. 이용언. 구자현. 윤진한.
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Abstract
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Nitric oxide(NO) has been known to be endothelial derived relaxing factor(EDRF) and nonadrenergic-noncholinergic(NANC) neurotransmitter involved in the relaxation of cavernous smooth muscle. This study was initiated to characterize NANC
neurotransmission by electrical field stimulation (EFS) and cholinergic neurotransmission by acetylcholine(Ach). Using organ bath, isometric tension measurements were made in strips of rabbit corpus cavernous smooth muscle.
@ES The results were as follows;
@EN 1. The relaxant response elicited by exogenous NO was very similar to the relaxation evoked by EFS via NANC pathway. Tetrodotoxin (1μM) nearly inhibited the relaxant response of EFS and Ach-induced relaxation was almost abolished in strips
which
was denuded endothelium.
2. L-NOARG (30μM, 100μM) significantly inhibited the relaxant effect of Ach and EFS, respectively, but that of exogenous NO was not influenced by this drug. L-arginine (300μM, 1mM) reversed the relaxant effect of Ach and EFS which were
inhibited by
L-NOARG.
3. Hemoglobin (60μM) significantly inhibited the relaxant response of EFS and exogenous No, and methylene blue (30μM) inhibited the relaxant response of Ach.
Above results suggest that NO is main neurotransmitter by NANC neurotransmission and EDRF by Ach, and effectively relax isolated rabbit cavernous smooth muscle is associated with an increae cGMP.
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